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cdaS [2020-04-01 14:43:52]
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cdaS [2020-04-01 14:43:52]

sporulation-specific diadenylate cyclase, synthesis of c-di-AMP
locus
BSU_19430
pI
5.37
mw
22.75 kDa
protein length
207 aa Sequence Blast
gene length
624 bp Sequence Blast
function
synthesis of c-di-AMP
product
sporulation-specific diadenylate cyclase
essential
no
ec
2.7.7.85
synonyms
yojJ
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.3|Metabolism of signalling nucleotides]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

    • Gene

    Coordinates
    2,118,504 2,119,127

    Phenotypes of a mutant

  • a [gene|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA] [gene|92EF98B872C1AD729B0BFAD46D3FA572E9C2AA32|cdaS] [gene|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA] triple mutant is not viable on complex medium; however, the mutant grows at low potassium concentration (0.1 mM) [pubmed|28420751]

    • The protein

    Catalyzed reaction/ biological activity

  • synthesis of c-di-AMP from two molecules of ATP [Pubmed|23192352]
  • 2 ATP --> 3',3'-c-di-AMP + 2 diphosphate (according to UniProt)

    • Protein family

  • adenylate cyclase family (with [protein|AFE2A9998219162FE32E95628B3CA9071099B61A|CdaA], according to UniProt)

    • Paralogous protein(s)

  • [protein|AFE2A9998219162FE32E95628B3CA9071099B61A|CdaA]

    • [SW|Domains]

  • N-terminal autoinhibitory domain [Pubmed|24939848]
  • C-terminal [SW|DAC domain] involved in the synthesis of c-di-AMP [Pubmed|21566650]
  • [SW|DAC domain] (aa 63-205) (according to UniProt)

    • Structure

  • [PDB|2FB5] (the protein of ''B. cereus'', 48% identity, 81% similarity)

    • Expression and Regulation

    Operons

    genes
    [gene|92EF98B872C1AD729B0BFAD46D3FA572E9C2AA32|cdaS]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|22383849], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

    • regulation

  • expressed late during sporulation in the forespore ([protein|search|SigG]) [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-B426 (yojJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1425 NBRP B. subtilis, Japan]
  • GP983 (''[gene|92EF98B872C1AD729B0BFAD46D3FA572E9C2AA32|cdaS]''::''ermC''), available in [SW|Jörg Stülke]'s lab [pubmed|23192352]
  • GP1360 (''[gene|92EF98B872C1AD729B0BFAD46D3FA572E9C2AA32|cdaS]''::''spc''), available in [SW|Jörg Stülke]'s lab
  • GP2222 ([gene|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA]::cat [gene|92EF98B872C1AD729B0BFAD46D3FA572E9C2AA32|cdaS]::ermC ''[gene|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA]''::''tet''), available in [SW|Jörg Stülke]'s lab, the mutant is only viable on minimal medium at low potassium concentration [pubmed|28420751]
  • BKE19430 ([gene|92EF98B872C1AD729B0BFAD46D3FA572E9C2AA32|cdaS]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCCTGTTTAAATTTCC, downstream forward: _UP4_TAAAAATAATAAAAAGGGCC
  • BKK19430 ([gene|92EF98B872C1AD729B0BFAD46D3FA572E9C2AA32|cdaS]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCCTGTTTAAATTTCC, downstream forward: _UP4_TAAAAATAATAAAAAGGGCC

    • Expression vectors

  • IPTG inducible expression of '''''B. cereus''''' Strep-''cdaS'' in ''E. coli'': pGP2593 (in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 25869574,18714086,23812326,30224435

    • Original publications

  • 21566650,23192352,22383849,24939848,26441857